Fig. 2.

Internalization and dissociation of NCP components in cells. a Uptake of chol-pyro-labelled NCP particles by CT26 cells at different incubation times. b The intracellular Pt in CT26 incubated with OxPt or OxPt/DHA as determined by ICP-MS. c CT26 cells incubated with fluorescent nanoparticles containing xylenol orange in the core, chol-pyro in the shell, and FITC-DOPE lipid to visualize OxPt in the core, chol-DHA in the shell, and the lipid layer of OxPt/DHA, respectively, for different times were observed under confocal laser scanning microscopy (CLSM). The experiments were done three times and data were expressed as mean ± SD in (a) and (b). The images in (c) were obtained without repetition. NCP nanoscale coordination polymer, OxPt oxaliplatin, DHA dihydroartemisinin, ICP-MS inductively coupled plasma-mass spectrometry