Fig. 7.

Hyperspectral imaging (HSI) of ex vivo human tissue from patients shows distinct spectral profiles. a Four representative synthesised RGB images of each sample. Dashed line indicates boundary of gastric mucosa, epithelium, submucosa, Barrett’s oesophagus and cancer, respectively. b–g Spectra of the identified tissue types shown in a. Solid lines and shaded areas in b–g indicate mean value and standard deviation of the absorbance profile, respectively. h Spectral angle mapper analysis using average spectral profile of oesophageal cancer as a reference signal. Error bars represent standard deviation. *** indicates p-value < 0.001. Statistical analysis was performed using a one-way ANOVA with post-hoc tests. All tissue was measured using the external illumination method. Scale bars = 1 mm