Fig. 4.

a BCL2 mRNA transcript stability determined by specific single-nucleotide variants changes. Relative transcript levels of GFP-BCL2 variants were measured by real-time qPCR (normalized to β-actin). b rs1801018 derived BCL2 produces a significantly more stable version of transcript, with an effect lasting 4 to 6 h post induction. c Protein levels of the variant BCL2 are higher than reference and random BCL2. All blots derive from the same experiment and were processed in parallel. d GFP protein levels were quantified compared with actin using ImageJ. e BCL2 RNAseq levels from 1417 patients with seven different types of cancer from, y-axis represents averaged FPKM levels, and the x-axis represents the SNP genotype. P-values were calculated using two-sided t test