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. 2019 Apr 23;9:6440. doi: 10.1038/s41598-019-42867-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pore clogging estimator b^eq for all residues Vs the amino acid volume V_a. Yellow circles corresponds to hydrophobic residues, green squares to polar, blue up-triangles to positively charged residues and red down-triangles to negatively charged ones. The dashed line is the minimum square fit. Panel a reports the b^eq calculated on the entire pore while panel b refers to the b^eq calculated removing the last part of the barrel including the secondary constriction, see the sketch in the inset. Error bars are estimated by considering b^eq from independent replicas as independent measurements and they are reported only when larger than symbols.