Fig. 3.

Shigella flexneri invades epithelia cells in a T3SS-dependent manner. a, b Representative images of colonic sections immuno-stained for E-cadherin and S. flexneri. a Merge: E-cadherin, green; S. flexneri, red. b S. flexneri only. Arrows indicate intracellular S. flexneri. Scale bars, 50 μm. c Zoom-in on the boxed area in a. d Graph showing counts of colony-forming units in the distal colon of animals infected with S. flexneri (wild-type (WT)) or the T3SS mutant (ΔmxiG). Statistical analysis, unpaired t test. ΔmxiG vs. WT, ***P < 0.0005. Source data are provided as a Source Data file