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. 2019 Apr 23;9:6434. doi: 10.1038/s41598-019-42703-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduction of granuloma number and volume after monocyte therapy. Liver sections sampled randomly and stained with Sirius red/fast green were examined by optical microscopy (100X magnification). Images of liver section of S. mansoni–infected mice 8 weeks after therapy with (A) vehicle, (B) Bone Marrow Mononuclear Cells (BMMCs) or (C) Monocytes, were digitalized and analyzed by morphometry for evaluation of (D) numerical density and (E) mean volume granulomas. Hepatic granulomas, well delimited and spherical shape which had the S. mansoni egg inside (black arrow) were considered during the measurement. Blue arrow – Schistosoma pigment. Values are presented as means ± S.E. (n = 6) *P < 0.05. Statistical analysis was performed by Kruskal-Wallis, followed by Dunn post-hoc test.