TABLE 1.
Bacterial strains, plasmids, and oligonucleotide primers used in this study
| Bacterial strain, plasmid, or primer |
Description or sequence | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH10B | F−
mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara leu)7697 araD139 galU galK nupG rpsL λ− |
New England Biolabs, UK |
| dam dcm mutant |
ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) Tets endA1 rspL136 (Strr) dam13::Tn9 (Camr) xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |
New England Biolabs, UK |
| C. jejuni strains | ||
| 11168H pglB::aphA | C. jejuni 11168H pglB is inactivated by aphA encoding kanamycin resistance cassette | This study |
| 11168H | Hypermotile variant of C. jejuni 11168 | 9 |
| 11168H cmeB::aphA | C. jejuni 11168H cmeB is inactivated by aphA encoding kanamycin resistance cassette | This study |
| 11168H napA::aphA | C. jejuni 11168H napA is inactivated by aphA encoding kanamycin resistance cassette | This study |
| Plasmids | ||
| pJMK30 | aphA gene cloned in BamHI restriction site | 41 |
| pRSA | cmeB amplicon cloned in pJET1.2 | This study |
| pRSF | aphA gene cloned in BamHI to inactivate cmeB in pRSA | This study |
| pATT3 | napA cloned in pJET1.2 | This study |
| pATT3F | aphA cloned in ClaI site inactivates napA in pATT3 | This study |
| Oligonucleotides | ||
| FWDcmeB | GACGTAATGAAGGAGAGCCA | |
| REVcmeB | CTGATCCACTCCAAGCTATG | |
| FWDnapA | ACCGCTATTGCAAGTGCTGCTAG | |
| REVnapA | GAAAGCGGACAAGTCGCATCC | |
| RTFWDPglA | ATG GGC AAA AAT TCC TTA TCG TTT | |
| RTREVPglA | GCT CGA CTA TAT CAC TTC TAG | |
| RTFWDPglC | CTC CGG TGA TTT TAA TCA CTG | |
| RTREVPglC | TTG CTT ACC CCA CTT CGT TT | |
| RTFWD PglJ | TTA GGA AGT GGT GGT GCT GA | |
| RTREV PglJ | AAA GCT ATG TCT TCA AGG GC | |