FIG 3.

Rv0565c expression alters ETH susceptibility in H37Rv. (a) Growth of strains overexpressing Rv0565c wild-type or SNP-containing alleles in various concentrations of ETH as measured by alamarBlue reduction. Each point represents the mean and standard deviation from three technical replicates, and the line represents the nonlinear regression of growth dependent on concentration of antibiotic. (b) Comparison of the growth of H37Rv (+) and the H37Rv deletion of Rv0565c (Δ) at various concentrations of ETH. Each point represents the mean growth from three cultures within an experiment with each of four experimental replicates connected by a line. The number below each set of points is the average ratio of deletion over wild type across the 4 experiments. *, P < 0.05; **, P < 0.01, two-tailed paired t test comparing growth at each concentration.