FIG 5.

Rv0565c expression more substantially alters PTH susceptibility. (a) Growth of strains overexpressing Rv0565c wild-type or SNP-containing alleles in various concentrations of PTH as measured by alamarBlue reduction. Each point represents the mean and standard deviation from three technical replicates, and the line represents the nonlinear regression of growth dependent on concentration of antibiotic. (b) Growth of H37Rv and strain 631 with their corresponding Rv0565c deletion mutants in various concentrations of PTH. Bar height represents the mean from three independent cultures with error bars showing standard deviations. The number above the bars is the ratios of the average of mutant over wild-type OD₆₀₀. Data are representative of two experiments. *, q < 0.05; **, q < 0.01; ***, q < 0.001; two-tailed t tests with false-discovery rate correction for multiple tests.