FIG 6.

Rv0565c deletion in strain 631 confers a long-term growth benefit in ETH and PTH. (a) Growth of a pooled mixture of strain 631 and 631 Rv0565c deletion mutant in the indicated medium as measured by OD₆₀₀. Strains were rediluted to 0.005 every 6 days. (b) The ratio of Rv0565c mutant over wild-type strain 631 over the course of treatment normalized to the starting library ratio on day 0. Each point represents the mean value from three qPCR technical replicates of three independent cultures with error bars showing the standard deviations among the three cultures. Each time point was compared with the input ratio by multiple t tests after two-way ANOVA and corrected for multiple hypothesis testing. ***, q < 0.001.