FIG 5.

PKR is not depleted by lysosomal degradation during MAV-1 infection. (A) CMT93 cells were infected with MAV-1 (MAV) at an MOI of 10 or were mock infected (mock) and treated with 10 mM ammonium chloride or 60 µM chloroquine to inhibit lysosomal degradation (or with water as a control). Cell lysates were analyzed by immunoblotting with antibodies for PKR (D-20) and actin. Blots are representative of results from three independent experiments. (B) Inhibitors were tested for activity using a DQ BSA assay; the DQ BSA molecule fluoresces only if lysosomal degradation is functional. Uninfected cells were treated as indicated and imaged by fluorescence microscopy.