FIG 7.

PKR is actively depleted early in infection. (A) CMT93 cells were infected with MAV-1 (MAV) at an MOI of 10 or were mock infected (mock) (top), or uninfected cells were treated with 50 µg/ml cycloheximide (CHX, bottom) to inhibit elongation of protein synthesis. Cell lysates were analyzed by immunoblotting with antibodies for PKR (D-20) and actin. Blots are representative of results from five independent experiments. (B) Densitometry quantitation of five independent experiments; **, P ≤ 0.01. (C) CMT93 cell lysates from the experiment performed as described in the panel A legend were analyzed with a second immunoblotting step with antibodies for E1A and actin. Blots are representative of results from four replicates from two independent experiments. The E1A blot image was uniformly adjusted to a brightness value of 30 and a contrast value of 5 in Adobe Photoshop.