Fig. 4.

(A) and (B) Intracellular uptake of ZnO and ZnO-PBA NPs by FACS and fluorescence microscopy analysis in MCF-7 cells. (C) MTT cell viability. (D) Phase contrast micrographs obtained at 20× magnification. (E) FACS analysis with annexin V staining to determine the percent of apoptotic cells in the different experimental groups. The cell in the lower left quadrant denotes annexin V positive or apoptotic cells. (F) and (G) Determination of intracellular ROS levels and MMP by FACS analysis, respectively.