FIG 7.

Reduced immune response to Clp mutants. WT C57BL/6J mice were infected subcutaneously with WT S. aureus and clp mutants for 5 days before punch biopsies were performed and samples were homogenized. (A) H&E-stained sections. Bars = 100 μm. UN, uninfected. (B and C) Clarified homogenized samples were used to quantify cytokine levels (B) and myeloperoxidase activity (C). Graphs show means with standard deviations (n = 12 for the WT and 8 for clp mutants, except for TNF [n = 7 for the clpP mutant]). ***, P < 0.001; **, P < 0.01; *, P < 0.05 (relative to the WT control).