FIG 7.
IL-10 produced by BM-MDSCs modulates lung injury during KP35 pneumonia. BM-MDSCs from IL-10−/− and IL-10/GFP VertX mice were generated in vitro. One day before the infection (day −1), 1 × 106 BM-MDSCs were transferred intranasally into IL-10−/− mice. At day 0 each group was intranasally infected with 1 × 108 CFU of KP35; at 48 h postinfection, mice were euthanized and lung histopathology was assessed through H&E staining (A). Lung injury was quantified through a histopathological score (B) and through the quantification of total proteins in the BALF (C). *, P < 0.05; ns, not significant, for a comparison of results for each parameter (by one-way ANOVA with a Tukey posttest).