TABLE 1.
Strains used in this study
| Strain | Description | Designation | Source or reference |
|---|---|---|---|
| AH-1263 | S. aureus USA300 strain LAC, plasmid cured | LAC (WT) | 91 |
| DH5α | E. coli strain for recombinant plasmids | DH5α | |
| BH10C | E. coli strain that restricts the plasmid copy number for complementation of mroQ | BH10C | 92 |
| RN4220 | Restriction-deficient S. aureus | RN4220 | 96 |
| RN9011 | RN4220/pRN7023 expressing SaPI1 integrase | RN9011 | 62 |
| FA-S922 | AH-1263 with an in-frame deletion of mroQ | ΔmroQ mutant | This work |
| FA-S982 | FA-S922 complemented with pJC1112-mroQ | ΔmroQ + mroQ mutant | This work |
| FA-S1008 | AH-1263 containing a gene replacement of agrBDCA with tetracycline resistance cassette | Δagr::tet mutant | This work |
| FA-S995 | FA-S922 transduced with Δagr::tet mutation | ΔmroQ Δagr::tet mutant | This work |
| FA-S1732 | AH-1263 with an in-frame deletion of agrB | ΔagrB mutant | This work |
| FA-S1736 | FA-S922 containing an in-frame deletion of agrB | ΔagrB ΔmroQ mutant | This work |
| AH-E462 | E. coli containing reporter plasmid pDB59 with gfp expression driven by the P3 promoter | 66, 97 | |
| FA-S945 | FA-S922 with GFP reporter plasmid pDB59 | ΔmroQ/pDB59 mutant | This work |
| FA-S984 | LAC Δagr with GFP reporter plasmid pDB59 | Δagr/pDB59 mutant | This work |
| FA-S1972 | FA-S1732 with GFP reporter plasmid pDB59 | ΔagrB/pDB59 mutant | This work |
| FA-S1973 | FA-S1736 with GFP reporter plasmid pDB59 | ΔagrB ΔmroQ/pDB59 mutant | This work |
| FA-S1145 | AH-1263 containing pOS1-PsarA-sodRBS-agrD-6×His | WT/pOS1-PsarA-agrD-6×His | This work |
| FA-S1146 | FA-S922 containing pOS1-PsarA-sodRBS-agrD-6×His | ΔmroQ/pOS1-PsarA-agrD-6×His mutant | This work |
| FA-S1147 | FA-S1008 containing pOS1-PsarA-sodRBS-agrD-6×His | Δagr/pOS1-PsarA-agrD-6×His mutant | This work |
| FA-S1265 | FA-S982 containing pOS1-PsarA-sodRBS-agrD-6×His | ΔmroQ + mroQ/pOS1-PsarA-agrD-6×His mutant | This work |
| FA-S1841 | FA-S922 containing pJC1111-mroQ(E141A) | ΔmroQ + mroQ(E141A) mutant | This work |
| FA-S1898 | FA-S922 containing pJC1111-mroQ(E142A) | ΔmroQ + mroQ(E142A) mutant | This work |
| FA-S1837 | FA-S922 containing pJC1111-mroQ(H180A) | ΔmroQ + mroQ(H180A) mutant | This work |
| FA-S2012 | FA-S1008 containing pOS1-PHELP-agrBD | Δagr/pOS1-PHELP-agrBD mutant | This work |
| FA-S2013 | FA-S995 containing pOS1-PHELP-agrBD | Δagr ΔmroQ/pOS1-PHELP-agrBD mutant | This work |