TABLE 2.

Oligonucleotides used in this study

Name	Sequence
MroQ-1	CCC-GGTACC(KpnI)-CCATAAATGATAAACCTCCAT
MroQ-2	GTGTGATTCGTTTTTTTTATTA-GGCGCC(KasI)-CATAATTTTCCTCCAAATATT
MroQ-3	AATATTTGGAGGAAAATTATG-GGCGCC(KasI)-TAATAAAAAAAACGAATCACAC
MroQ-4	CCC-GAGCTC(SacI)-ATTTTTAGCCTTGGCAAATG
37F	GGCGGATCCAGCACTATCAGTTAAAACAAT
37R	GGCGAATTCACAAGAAGATAATAAGAAAAG
agrB-1	ATAT-GGTACC(KpnI)-TAACTCTACTAGCAAATGTTACTC
agrB-2	GTTAAATAATGTATTCATTTTTACACCACTCTCCTCA
agrB-3	TGAGGAGAGTGGTGTAAAAATGAATACATTATTTAAC
agrB-4	ATAT-GAGCTC(SacI)-TATTACAAATATACTATCAGAAATTTTGGTG
MroQPM-CompF	GGC-CTGCAG(PstI)-AGCACTATCAGTTAAAACAAT
MroQPM-CompR	GGC-TCTAGA(XbaI)-ACAAGAAGATAATAAGAAAA
MroQ(H180A)-2	AAATTTGAAATCATTGGCTGCTAATGCAAATATTAATG
MroQ(H180A)-3	CATTAATATTTGCATTAGCAGCCAATGATTTCAAATTT
MroQ(E141A)-2	TTTTCTGAATACATATTCTGCTAATAAAGGACCAATAA
MroQ(E141A)-3	TTATTGGTCCTTTATTAGCAGAATATGTATTCAGAAA
MroQ(E142A)-2	TTTTCTGAATACATATGCTTCTAATAAAGGACCAATAA
MroQ(E142A)-3	TTATTGGTCCTTTATTAGAAGCATATGTATTCAGAAAA
phelp-agrBD-1	ATAT-GTCGAC(SalI)-ATCCCATTATGCTTTGGCA
phelp-agrBD-2	CAATTTTATTATCAAAATAATTCAAGGGTTTCACTCTCCTTCTA
phelp-agrBD-3	TAGAAGGAGAGTGAAACCCTTGAATTATTTTGATAATAAAATTG
phelp-agrBD-4	ATAT-GAATTC(EcoRI)-TTATTCGTGTAATTGTGTTAAT
PsarA-AgrD6×His-1	ATAT-CTGCAG(PstI)-CTGATATTTTTGAC
PsarA-AgrD6×His-2	AAATAAGTTAAATAATGTATTCAT-AAATAATCATCCTCCTAAGG
PsarA-AgrD6×His-3	CCTTAGGAGGATGATTATTT-ATGAATACATTATTTAACTTATTT
PsarA-AgrD6×His-4	ATAT-GAATTC(EcoRI)-TTAGTGATGGTGAATGGTGA

^aUnderlining and boldface indicate locations of nucleotide substitutions.