FIG 1.

Timing of parasite egress and SUB1 expression. Viable Cryptosporidium parvum sporozoites were used to infect HCT-8 monolayers. (A) Supernatants were collected and assayed for released parasites using qPCR. The peak count of released merozoites was observed at 24 h postinfection. Data are representative of the results of four independent experiments. **, P < 0.0001; *, P < 0.005. (B) Sporozoite-infected HCT-8 monolayers were collected at different times postinoculation. Levels of SUB1 mRNA were analyzed by qRT-PCR and relative quantification. Peak expression of SUB1 was observed at 12 to 20 h postinfection with respect to initial infection at 1 h (P < 0.0001). Data are representative of the results of three independent experiments.