FIG 2.

Design of ssRNA guides and inhibition of SUB1. (A) SUB1 ssRNA and SUB1 mRNA secondary structure. (B) Silencing was carried out in oocysts, and levels of silencing were determined by RT-PCR. With guide sequences 1 and 3, parasites were not detectable (n.d.). Sequence 2 failed to silence the expression of SUB1. Guide sequence 5 demonstrated moderate silencing, while sequence 4 showed significant silencing (P < 0.0001). Data are representative of the results of three independent experiments.