FIG 2.

IFN-λ treatment does not affect antimicrobial peptide expression in the lung. Mice were infected with 25 PFU influenza virus A/PR/8/34 H1N1 or PBS as a control and 5 days later given 1 × 10¹⁰ VP of Ad-IFN-λ or Ad-GFP. Twenty-four hours following adenoviral treatment, mice were challenged with 5 × 10⁷ CFU MRSA and harvested 1 day later. (A) Whole lung lobes were snap-frozen in liquid nitrogen, and RNA was extracted and assayed by real-time PCR for expression of IL-22 and the antimicrobial peptides ngal, reg3g, s100a8, s100a9, and camp (n = 4; two to three independent experiments). (B) Bronchoalveolar lavage fluid was centrifuged to pellet cells. The supernatant was removed, and the cell pellet was frozen at −80°C for RNA extraction and subsequent real-time PCR. FS, MRSA infection preceded by influenza virus infection for 6 days. ns, not significant.