FIG 7.

hAMs secrete substantial levels of the chemokine IL-8 in response to C. burnetii. hAMs from three separate donors were infected with C. burnetii for 2, 24, 48, or 72 h. At each time point, supernatants were harvested and assessed for the presence of IL-8 via ELISA in triplicate. UI, uninfected cell supernatants. Supernatants from cells treated with LPS and ATP served as a positive control. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. All donor hAMs display significant IL-8 secretion during C. burnetii infection.