Table 2.
Screening of potential ORFs from strains L11 and S1-5 using the reported AHL quenching enzymes.
| Strain name | ORF name | Protein size (kDa) | Signal peptide prediction | Asp + Glu (%) | Hypothetical function | Superfamily | References QQ ORF | Coverage | Identity | E-value |
|---|---|---|---|---|---|---|---|---|---|---|
| S1-5 | AiiAS1-5 | 29.6 | √ | 13.2 | N-acyl homoserine lactonase (AiiA) | Metallo-hydrolase-like_MBL-fold superfamily | AiiA Bacillus thuringiensis (Liu et al., 2008) | 84% | 26% | 2e-24 |
| SDRS1-5 | 25.3 | – | 10.2 | NAD(P)-dependent oxidoreductase (SDR) | Short-chain dehydrogenase/reductase (SDR) family | BpiB09 soil metagenome (Bijtenhoorn et al., 2011) | 95% | 41% | 1e-45 | |
| EstS1-5 | 31.8 | √ | 9.9 | Esterase (Est) | α/β hydrolase family | dlhR Rhizobium sp. NGR234 (Krysciak et al., 2011) | 45% | 36% | 4e-07 | |
| HydS1-5 | 38.3 | – | 11.9 | α/β hydrolase (Hyd) | α/β hydrolase family | AidH Ochrobactrum sp. (Mei et al., 2010) | 49% | 28% | 1E-11 | |
| L11 | HydL11 | 28.8 | – | 13.3 | α/β hydrolase (Hyd) | α/β hydrolase family | Est816 soil metagenome (Fan et al., 2012) | 83% | 25% | 1e-16 |
| PvdQL11 | 83.8 | – | 11.3 | Penicillin acylase family protein (PvdQ) | Ntn_hydrolase superfamily | QuiP P. aeruginosa PAO1 (Huang et al., 2006) | 96% | 25% | 2e-43 | |
| SDRL11 | 26.8 | – | 12.0 | Oxidoreductase (SDR) | SDR family NAD(P)-dependent oxidoreductase | BpiB09 soil metagenome (Bijtenhoorn et al., 2011) | 98% | 32% | 6e-42 | |
| HPL11 | 36.0 | – | 10.8 | Hypothetical protein (HP) | DUF523 and DUF1722 domain-containing protein | QQ-16d Pseudoalteromonas sp. (Weiland-Bräuer et al., 2015) | 99% | 48% | 8e-106 | |
Potential QQ ORFs were screened by tBLASTn using known QQ enzymes published as references.