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. 2019 Apr 17;10:847. doi: 10.3389/fmicb.2019.00847

Table 2.

Comparisons of the abundances of SLP enzymes, ETC components, and NADH oxidases during glucose fermentation and of the relative abundance of corresponding species between groups.

PATHWAY ENZYME GENE ABUNDANCE
 SPECIES RELATIVE ABUNDANCE
G1 P1 B1 G/B2 P/B2 G1 P1 B1 G/B2 P/B2
SLP enzymes Glycolysis EC 2.7.2.3; pgk; phosphoglycerate kinase 1490.58 ± 76.36 1097.76 ± 26.08 1158.74 ± 67.61   0.36 –0.08 1308.5 ± 112.3 1276.4 ± 93.1 1823.2 ± 115.4 –0.48 –0.51
EC 2.7.1.40; pyk; pyruvate kinase   589.59 ± 25.46   480.02 ± 39.72   436.98 ± 43.85   0.43   0.14   846.5 ± 57.9   751.5 ± 35.9   718.2 ± 37.2   0.24   0.07
Pyruvate/Propanoate metabolism EC 2.7.2.1; ackA; acetate kinase     910.36 ± 120.20   625.56 ± 57.83   749.60 ± 63.99   0.28 –0.26   2163.9 ± 185.2   1326.4 ± 111.9   1669.2 ± 137.2   0.37 –0.33
Butanoate metabolism EC 2.7.2.7; buk; butyrate kinase   980.68 ± 41.05   410.10 ± 64.37     264.02 ± 130.54   1.89*   0.64 1320.1 ± 99.3   799.1 ± 74.4 518.3 ± 46.7 1.35*   0.62
Nitrogen metabolism EC 2.7.2.2; arcC; carbamate kinase   202.21 ± 10.26   174.72 ± 46.94   152.81 ± 28.83   0.40   0.19   479.8 ± 24.5   448.3 ± 32.0  431.3 ± 32.1   0.15   0.06
NADH oxidases in glucose fermentation Heterolactic fermentation EC 1.2.1.10; adhE; acetaldehyde dehydrogenase   663.37 ± 20.90   208.92 ± 20.27   227.83 ± 10.88   1.54* –0.13     1.7 ± 0.5     0.3 ± 0.2     1.1 ± 0.1   0.68 –1.79*
EC 1.1.1.1; adhP; alcohol dehydrogenase   14.74 ± 2.87     4.75 ± 1.14     4.29 ± 1.33   1.78*   0.15   436.7 ± 22.7   460.2 ± 34.1   632.7 ± 68.3 –0.53 –0.46
EC 1.1.1.1; adh; alcohol dehydrogenase     8.15 ± 1.77     5.66 ± 1.65     3.87 ± 1.31   1.07*   0.55   32.8 ± 4.5   28.2 ± 3.1     9.9 ± 1.1   1.73*   1.51*
EC 1.1.1.1; adhC; alcohol dehydrogenase   16.55 ± 6.07     2.01 ± 1.40     6.49 ± 1.48   1.35* –1.69*     8.4 ± 1.1     4.5 ± 0.6     8.4 ± 0.4 –0.01 –0.89
EC 1.1.1.1; yiaY; alcohol dehydrogenase   121.40 ± 10.44   32.83 ± 5.67   38.95 ± 7.32   1.64* –0.25    16.8 ± 1.2     7.0 ± 1.1    11.0 ± 0.5   0.61 –0.67
EC 1.1.1.2; adh; alcohol dehydrogenase (NADP+)     79.88 ± 11.90     81.79 ± 17.51     78.46 ± 18.96   0.03   0.06    53.6 ± 4.2   94.3 ± 9.7   79.0 ± 4.1 –0.56   0.26
EutG; alcohol dehydrogenase   40.05 ± 5.12   13.59 ± 3.35   12.94 ± 3.21   1.63*   0.07   116.1 ± 6.8 164.8 ± 9.5 217.6 ± 5.8 –0.91 –0.40
Homolactic fermentation EC 1.1.1.27; ldh; L-lactate dehydrogenase   118.76 ± 33.54   125.58 ± 27.77     86.46 ± 15.35   0.46   0.54   125.6 ± 5.2 128.1 ± 5.9 140.9 ± 7.7 –0.17 –0.14
Butanol fermentation EC 1.3.1.44; ter; trans-2-enoyl-CoA reductase (NAD+)     0.62 ± 0.01     0.62 ± 0.01     0.97 ± 0.00 –0.64 –0.64   725.3 ± 54.7   962.8 ± 68.5   703.4 ± 55.6   0.04   0.45
EC 1.1.1.-; bdhAB;butanol dehydrogenase   833.56 ± 48.40   414.17 ± 20.41   451.46 ± 20.64   0.88 –0.12   436.7 ± 22.7   460.2 ± 34.1   632.7 ± 68.3 –0.53 –0.46
EC 1.2.1.10; adhE; acetaldehyde dehydrogenase   663.37 ± 20.90   208.92 ± 20.27   227.83 ± 10.88   1.54* –0.13     1.7 ± 0.5     0.3 ± 0.2     1.1 ± 0.1   0.68 –1.79
Propionate fermentation EC 1.1.1.37; mdh; malate dehydrogenase   612.69 ± 54.98   415.07 ± 96.21   383.46 ± 55.67   0.68   0.11   62.9 ± 3.8   33.6 ± 3.6   34.5 ± 3.2   0.86 –0.04
ETC components ATPase ATPase; F-type H+-transporting ATPase   768.75 ± 76.10   382.62 ± 57.24   353.58 ± 41.08   1.12*   0.11   1926.0 ± 197.8   1629.1 ± 187.1   1601.6 ± 192.2   0.27   0.02
FADH2-NAD oxidoreductase3 Rnf; electron transport complex protein Rnf   910.04 ± 66.10   417.25 ± 67.79   443.12 ± 51.49   1.04* –0.09   2295.6 ± 191.3   1574.6 ± 149.0   1424.0 ± 120.8   0.69   0.15
Methanogenesis Mtr; tetrahydromethanopterin S-methyltransferase     2.20 ± 0.74     1.96 ± 0.29     2.09 ± 0.46   0.07 –0.09     7.0 ± 0.8     4.3 ± 0.5     6.8 ± 1.3   0.04 –0.65
NADH dehydrogenase3 Nuo; NADH-quinone oxidoreductase   230.44 ± 22.47   120.09 ± 20.18   113.48 ± 11.59   1.02*   0.08   2253.7 ± 176.9   1378.7 ± 155.5  1014.8 ± 73.2   1.15*   0.44
Ndh; NAD(P)H-quinone oxidoreductase   13.78 ± 3.49   12.97 ± 3.49   10.58 ± 2.85   0.38   0.29   20.0 ± 2.1     8.2 ± 1.2   12.2 ± 0.6   0.71 –0.57
Hox; bidirectional [NiFe] hydrogenase diaphorase   13.74 ± 3.20     7.52 ± 1.14   10.89 ± 2.14   0.34 –0.53     5.2 ± 0.7     2.1 ± 0.3     5.8 ± 0.6 –0.16 –1.45*
Cytochrome complex3 Pet; ubiquinol-cytochrome c reductase     0.71 ± 0.21     0.89 ± 0.14     0.63 ± 0.11   0.16   0.51 0 0 0   0   0
TorC; trimethylamine-N-oxide reductase     0.47 ± 0.03     0.83 ± 0.04      0.65 ± 0.07 –0.45   0.36     5.4 ± 1.1     2.8 ± 0.6     3.8 ± 0.4   0.50   –0.47
Cyd; cytochrome bd ubiquinol oxidase   412.15 ± 47.13   539.51 ± 33.78   366.26 ± 50.13   0.17   0.56   2035.6 ± 212.7   1044.0 ± 116.0   911.6 ± 68.2   1.16*   0.2
Sulfur dissimilation Apr; adenylylsulfate reductase     0.64 ± 0.06     0.59 ± 0.05      0.78 ± 0.31 –0.28 –0.39     2.8 ± 0.4   19.9 ± 2.1   24.6 ± 2.0 –3.13* –0.31
Nitrogen dissimilation NrfA; nitrite reductase (cytochrome c-552)   403.53 ± 65.93   202.95 ± 29.52   176.38 ± 24.09   1.19*   0.20   541.3 ± 45.7   337.5 ± 40.5   259.7 ± 22.2   1.06*   0.38
Nar; nitrate reductase / nitrite oxidoreductase     0.55 ± 0.09     1.60 ± 0.15     1.72 ± 0.22 –1.63* –0.10     0.2 ± 0.1     8.5 ± 0.9     3.1 ± 0.2 –3.90*   1.46*
NapA; periplasmic nitrate reductase NapA     0.82 ± 0.03     1.89 ± 0.12     0.83 ± 0.31 –0.02   1.20*     0.34 ± 0.13     4.62 ± 1.08     1.56 ± 0.28 –3.76*   1.57*
Fumarate respiration Sdh; succinate dehydrogenase  1210.06 ± 130.02   578.79 ± 32.16   500.64 ± 64.78   1.27*   0.21   1422.9 ± 114.3 1179.1 ± 95.8   976.6 ± 79.6   0.54   0.27

1 mean value ± standard error; the unit of gene results is RPKM; the relative abundance of species results was normalized to 1,000,000 for each sample. 2 the numbers were calculated from log2(G/B) or log2(P/B). 3 the electron transporters were reported in many kinds of ETCs. ∗p < 0.05 and | value| > 1 in the wilcox test between groups.