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. 2019 Apr 17;10:349. doi: 10.3389/fphar.2019.00349

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Copyright © 2019 Mercurio, Sharples, Corbo, Franchini, Vacca, Catalano, Carocci, Kamm, Pavesi and Adriani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of the teratogenic side effects in zebrafish embryos. (A) Malformations were measured by three teratogenic parameters in the zebrafish embryo: the eye area (A), the pectoral fin length (B) and the ratio between the long axis of the otic vesicle and the body length (C). The eye area (A) and the pectoral fin (B) were measured at 75hpf and the otic vesicle (C) was measured at 30hpf for optimal visualization. Lateral view of embryo eye (A), dorsal views of pectoral fins (red arrowheads in B) and lateral view of the otic vesicle for each condition are showed. For each parameter, the data are shown as percentage variation in respect to the treatment with vehicle. The data are plot in a scattered dot plot showing the mean ± SD. Statistics were calculated by one-way ANOVA. n.s. = not significant; *p < 0.05; **p < 0.01; ****p < 0.0001.