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Use of 96-well plate blocker to remove out of well transmitted light scatter from wells. (A) Top left and right: Montage images of a 96-well plate before and after plate blocker usage. Center: image of plate blocker used. Bottom left and right: images of wells captured before and after blocker. (B) Comparison of automated image acquisition modalities from a 96-well plate; effects of laser autofocus on acquisition speed and colony count.
