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. 2019 Apr 4;39(4):233–245. doi: 10.1089/jir.2018.0129

FIG. 5.

FIG. 5.

The IL-12/MMPcs1/scFv1 FP is specifically cleaved by MMP9. The IL-12/scFv1 FPs containing either the MMPcs1 or the scrambled control sequence were incubated with active MMP9 or buffer control and the resulting material tested by western analysis. Immunoblot analysis of the digested samples using an anti-IL-12 detection antibody shown on the left (A) or an anti-6x His detection antibody, which detects the carboxy terminal 6x His tag on the scFv is shown on the right (B). Molecular weight standard is shown on left. Note that the + and − MMP9 digests were run on the same gel and exposed for the same amount of time but some intervening lanes were removed.