FIG. 1.

STC1 is decreased in TLR4^−/− MLECs and mice. (A) Affymetrix microarray analysis for STC1 in primary isolated MLECs (experiments were performed in triplicate). Data are expressed as mean in the fold of change and p-value and analyzed by unpaired Student's t test, multiple testing correction with the method of Benjamini–Hochberg, TLR4^−/− vs WT MLECs. (B) STC1 mRNA expression in WT or TLR4^−/− MLECs was measured by real-time RT-PCR and analyzed by the Mann–Whitney test. *p < 0.05 vs WT (experiments were performed in triplicate). (C) Total lung lysates isolated from 3-month-old WT or TLR4^−/− mice were immunoblotted against STC1 antibody. β-Actin was used as protein loading control. See Supplementary Figure S9A for uncut gel. MLECs, mouse lung endothelial cells; RT-PCR, reverse transcription-polymerase chain reaction; STC1, stanniocalcin 1; TLR4, toll-like receptor 4; WT, wild type.