Table 2:
Read characteristics of processed RNA-Seq data
| SRA Run ID | Read length | No. of reads per strand | BioSample ID | Sample description | Sequencing instrument |
|---|---|---|---|---|---|
| SRR4250750 | 50 | 1,000,425 | SAMN05779985 | Cultured embryonic stem cells | Illumina HiSeq 2500 |
| SRR5185518 | 50 | 5,008,398 | SAMN06239034 | Cultured embryonic stem cells | Illumina HiSeq 2500 |
| SRR2932901 | 50 | 10,017,495 | SAMN04211783 | Fetal lung fibroblasts | Illumina HiSeq 2500 |
| SRR4428678 | 75 | 1,043,870 | SAMN05913930 | Embryonic stem cell−derived macrophage | Illumina HiSeq 4000 |
| SRR4241930 | 75 | 5,004,985 | SAMN05770731 | Primordial germ cell−like cells | Illumina HiSeq 2000 |
| ERR204893 | 75 | 10,234,883 | SAMEA1573291 | Lymphoblastoid cell line | Illumina HiSeq 2000 |
| SRR5168756 | 100 | 1,006,868 | SAMN06218220 | Subcutaneous metastasis | Illumina HiSeq 2500 |
| SRR5023408 | 100 | 5,004,554 | SAMN06017954 | Primary breast cancer | Illumina HiSeq 2500 |
| SRR2567462 | 100 | 10,007,044 | SAMN04147557 | Prostate cancer cells LNCaP | Illumina HiSeq 2500 |
We chose 9 different RNA-Seq datasets from the Sequence Read Archive (SRA), a public high-throughput sequencing repository. To compare their performance, each sequence was selected to be different in terms of read length and total number of reads. All data are from human samples sequenced by the Illumina HiSeq platform.