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. 2019 Mar 31;20(7):1604. doi: 10.3390/ijms20071604

Figure 1.

Figure 1

Phenotypic characterization of DECs and ADMECs. (A) Immunofluorescence analysis of vWF, VE-cadherin and vimentin on isolated and cultured DECs and ADMECs. Original magnification: 200×. (B) RT-qPCR of VEGF-A, HGF, IGFBP3, ICAM-2 and ICAM-3 genes differentially expressed by DECs and ADMECs. The data represent the mean ± SD of triplicate samples from five separate experiments, * p < 0.05, ** p < 0.01, *** p < 0.005. (C) Evaluation of the production of VEGF-A, HGF and IGFBP3 proteins in the supernatants of a confluent monolayer of DECs and ADMECs after 4 h of culture using a commercial ELISA kit. The data represent the mean ± SD of triplicate samples from five separate experiments. * p < 0.05, ** p < 0.01, *** p < 0.005. (D) Cytofluorimetric analysis for the expression of ICAM-2 and ICAM-3 in basal condition of freshly isolated DECs and ADMECs. The ECs were incubated with PE-conjugated mouse anti-human ICAM-2 and ICAM-3 mAb. PE-conjugated isotype-matched IgG2a or IgG1 were used as negative control, respectively. Data are represented as mean ± SD of the Mean Fluorescence Intensity (MFI) of five separate experiments.