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Permeabilizing activity of endothelial cells to classical vasoactive stimuli. The permeabilizing activity was evaluated kinetically, after 5 (A), 15 (B) and 30 min (C) adding PAF (D), HIS (E) or BK (F), to the upper chamber of the TW, measuring the amount of FITC-labeled BSA that leaked through a monolayer of endothelial cells into the lower chamber. The data represent the mean ± SD of duplicate samples from four separate experiments * p < 0.05; ** p<0.01; *** p < 0.005.
