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. 2019 Mar 31;20(7):1604. doi: 10.3390/ijms20071604

Figure 5.

Figure 5

Secretion of chemokines and trans-endothelial migration of LM through EC stimulated with IFN-γ. The production of CXCL10/IP-10 (A) and CXCL9/MIG (B) in DEC and ADMEC supernatants after 4 h incubation with IFN-γ was measured using a beads-based multiplex immunoassay (Luminex®). The data represent the mean ± SD of triplicate samples from five separate experiments * p < 0.01. (C,D) Trans-endothelial migration of LM across untreated and IFN-γ-treated DEC and ADMEC. (C) Representative dot plots for flow cytometry of trans-endothelial migrated LM, stained for CD3 and FoxP3. (D) Quantitation of the percent of total migrated LM cells, positive for both CD3 and FoxP3 (CD3+FoxP3+) by flow cytometry. Migration was presented as the percentage of CD45 migrated cells. The data represent the mean ± SD of duplicate samples from three separate experiments * p < 0.05.