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. 2019 Apr 10;20(7):1772. doi: 10.3390/ijms20071772

Figure 1.

Figure 1

Rhododendrin increases RNF146 expression. (A) Quantification of relative RNF146 promoter activity in SH-SY5Y cells transfected with pGL3-RNF146-Luc and pRL-TK for 24 h, followed by treatment with 10 µM of rhododendrin for 37 h (n = 6 per group). (B) Quantification of relative RNF146 mRNA levels normalized to GAPDH in SH-SY5Y cells treated for 37 h with 0.1, 1, and 10 µM of rhododendrin (n = 3 per group). (C) Representative Western blot showing RNF146 expression in SH-SY5Y cells treated for 37 h with the indicated concentrations of rhododendrin (0.1, 1, 10 µM). β-actin served as a loading control. (D) Quantification of relative RNF146 protein levels normalized to that of β-actin in SH-SY5Y cells treated for 37 h with 0.1, 1, and 10 µM of the rhododendrin (n = 3 per group). Data are expressed as mean ± SEM. ** p < 0.01 and *** p < 0.001, ANOVA test followed by Tukey’s post-hoc analysis.