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. 2019 Apr 8;20(7):1737. doi: 10.3390/ijms20071737

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inhibition of the hematopoiesis process by overexpression of miR-206 in a blood-competent ES cell line. The hematopoietic-competent H1 ES cell line was transduced with a lentivirus expressing either miR-206 (H1-206) or the ath-miR-159a negative control (H1-ath). (A) miR-206 average expression in H1-206 cells as compared to H1-ath cells. Fold changes were calculated by the 2^−ΔΔCt method with RNU48 as endogenous control; (B) The number of CFCs and the percentage of CD34+ cells in day-16 EBs (mean ± SD) are shown on the graphs for H1-ath control (blue bar) and H1-206 (red bar).