Figure 1.

(A) Corticosterone levels of wild-type (WT) and forebrain-specific mineralocorticoid receptor (MR) knockout (fbMRKO) mice. In these mice chromatin immunoprecipitation coupled with quantitative polymerase chain reaction (ChIP-qPCR) measurements for (B) glucocorticoid receptor (GR) and (C) Neurod2 were performed. For each gene, the corresponding immunoglobulin G (IgG) background signal is subtracted from detected binding levels, expressed as the percentage of immunoprecipitated DNA. The binding sites near Fkbp5, Klf9, Per1, Kif1c and Zfp219 are joint MR/GR loci, while Rilpl1 has been identified as an MR-specific target [9] (separated by the right dotted line). Genes are further sorted based on the absence (Fkbp5, Klf9, Per1) or presence (Kif1c, Zfp219, Rilpl1) of a NeuroD binding sequence near the MR binding site (separated by the left dotted line). *** p < 0.001