Figure 2.

Specificity of NeuroD coactivation at the previously identified binding motif (CAGATG) for (A) MR and (B) GR. HEK293 cells were transfected with GRE-At_GA luciferase construct, MR or GR (10 ng/well), various amounts of Neurod2, MyoD or the MyoD/Neurod2 chimera (MyoD(ND2bHLH)) (1–3–10 ng/well), and stimulated with corticosterone (10⁻⁷ M). Data are presented as luciferase activity fold induction upon corticosterone treatment. a.u. = arbitrary unit; ** p < 0.01, **** p < 0.0001 compared to control condition; # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 for within group comparisons