Figure 3.
Modulation by NeuroD and MyoD variants at the shared binding motif (CAGCTG) for (A) MR- and (B) GR-mediated transcription. HEK293 cells were transfected with GRE-At_GC luciferase construct, MR or GR (10 ng/well), and Neurod2, MyoD, MyoDΔN or Myf5 (10 ng/well), and stimulated with corticosterone (10−7 M). Luciferase activity of nonstimulated control cells was normalized to 1. Numbers represent fold induction upon corticosterone treatment. a.u. = arbitrary unit; * p < 0.05, ** p < 0.01, **** p < 0.0001 compared to control condition; #### p < 0.0001 compared to Neurod2 condition; && p < 0.01 compared to MyoD condition