Figure 3.

Representative time resolved UV-Vis spectra of the interaction of H₂S or GSH with SeO₃²⁻ or SeCl₄. The interactions of: (a) H₂S (100 µM) with SeO₃²⁻ (100 µM), (b) GSH (100 µM) with SeO₃²⁻ (100 µM), and (c) H₂S (100 µM) with SeCl₄ (100 µM). All samples were prepared and measured in 100 mM sodium phosphate, 100 µM DTPA buffer, pH 7.4, 37 °C. The spectra are represented in the form of solid and broken lines. The solid red line indicates the first spectrum after addition of H₂S or GSH to SeO₃²⁻ or. SeCl₄, which is followed by gradual broken red, solid blue and gradually broken blue line which represent the spectra recorded every 30 s.