Figure 4.

Fluorescence images of MCF7, A427 and A431 cells treated with different concentrations of bikaverin for 24 h. Cell nuclei were double stained by Hoechst 33342 (blue fluorescence), and by EdU-assay using 5-TAMRA-PEG3-azide as a coupled fluorophore (violet fluorescence). Proliferating cells were monitored by EdU-assay. The pictures are overlay of the fluorescence images of Hoechst-stained cells and TAMRA-labeled proliferating cells. The cells that are emitting only blue fluorescence are not proliferating, in contrast to those emitting an additional violet fluorescence. A Keyence microscope was used to obtain the pictures using a 40-fold lens.