Table 1.
Purification | Total Protein (mg) | Total Activity (U) | Specific Activity (U/mg) | Purification Fold | Yield (%) |
---|---|---|---|---|---|
Crude enzyme | 90.90 | 3876.1 | 42.64 | 1.0 | 100.0 |
Heat treatment | 25.05 | 3206.9 | 128.0 | 3.0 | 82.7 |
Ni-Sepharose | 10.08 | 2856.0 | 283.3 | 6.6 | 73.7 |