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Trypanosoma cruzi recombinant proteins Sir2rp1 and Sir2rp3 in vitro deacetylase activity. (A) SDS-PAGE stained with Coomassie brilliant blue of purified TcSir2rp1 and TcSir2rp3 recombinant proteins. (B) Schematic representation of the deacetylation assay used to test the TcSir2rp activity. The NAD+-deacetylase activity of TcSir2rp1 (C) and TcSir2rp3 (D) recombinant proteins. Bars represent the mean ± standard error of the mean (SEM) of three experiments. *** p < 0.001.
