. 2019 Apr 3;24(7):1299. doi: 10.3390/molecules24071299

Table 1.

Deacetylase (TcSir2rp1 and TcSir2rp3) and antiparasitic activity in trypomastigote, intracellular parasite, host cell cytotoxicity, and selectivity index of Anacardium occidentale chemical compounds.

Compounds	TcSir2rp1	TcSir2rp3	Trypomastigote	Amastigote	hFIB	SI ^e
Compounds	EC₅₀ ± SEM (µM) ^a	EC₅₀ ± SEM (µM) ^a	EC₅₀ ± SEM (µM) ^b	EC₅₀ ± SEM (µM) ^c	CC₅₀ ± SEM (µM) ^d	SI ^e
1	NA	NA	23.36 ± 0.12	11.75 ± 0.40	47.17 ± 0.10	4
2	31.40 ± 2.33	NA	12.25 ± 0.25	14.70 ± 3.27	53.70 ± 7.08	4
3	NA	NA	NA	46.98 ± 1.97	>100	>2
4	NA	NA	43.52 ± 2.32	15.28 ± 2.00	45.47 ± 0.27	3
5	23.35 ± 2.51	6.08 ± 1.16	NA	41.67 ± 5.53	>100	>2
6	16.16 ± 4.88	7.41 ± 0.65	NA	42.39 ± 5.11	>100	>2
Benznidazole			11.40 ± 1.09	3.13 ± 0.87	>100	>32

^a Sirtuin activity was determined 4 h after incubation. ^b Determined 24 h after incubation with compounds. ^c Infected human primary skin fibroblasts (hFIB) were exposed to compounds and activity was determined 72 h after treatment. ^d hFIB viability was determined 72 h after treatment. ^e Selectivity index (SI), calculated by the ratio of CC₅₀ (hFIB) to EC₅₀ (amastigote). EC₅₀, effective concentration at 50%; CC₅₀, cytotoxic concentration at 50%. ND, not determined due to lack of activity. Values were calculated using concentrations in triplicate, and three independent experiments were performed.