Figure 2.

Implementing a PLANT-Dx diagnostic. (a) PLANT-Dx reactions were used to detect CMV from infected N. tabacum derived lysate (orange) versus uninfected plant-derived lysate (gray), across three different days. Data displayed as in Figure 1b,c. (b) Time lapse photos of cell-free reactions containing RPA products from CMV infected lysate (+) or uninfected lysate (−). (c) Demonstration that PLANT-Dx can operate using only body heat as a heat source. RPA and cell-free reactions were run by taping tubes on the inner arm for 40 and 150 min, respectively. A yellow color was observed for the CMV-infected lysate sample (+) and not the uninfected lysate control (−). Data in (a) represent mean values, and error bars represent s.d. of n = 3 technical replicates.