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. 2019 Mar 28;20(7):1559. doi: 10.3390/ijms20071559

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of inhibition of AQP3 function on warfarin permeability. Caco-2 cells were plated in Transwell plates and incubated for 21 days. (A) Warfarin alone or a combination of warfarin and HgCl₂ was added to the apical side of the Transwell plate. (B) Warfarin alone or a combination of warfarin and HgCl₂ was added to the apical side of the Transwell plate, and mannitol was added to the basal side. (C) Warfarin alone or a combination of warfarin and HgCl₂ was added to the apical side, and the Transwell plate was shaken on a plate mixer. (D) Glycerol alone or a combination of glycerol and phloretin was added to the apical side of the Transwell plate. (E) Warfarin alone or a combination of warfarin and phloretin was added to the apical side of the Transwell plate. The concentration of warfarin or glycerol on the basal side was measured. The data are presented as the mean ± SD for six experiments.