Table 1.

P_app of glycerol during Caco-2 cell differentiation.

	Glycerol (10−6 cm/s)	Glycerol + HgCl2 (10−6 cm/s)
7 days	2380 ± 715	2172 ± 779
14 days	1986 ± 1222	929 ± 101 *
21 days	1000 ± 148	415 ± 66 **

Caco-2 cells were plated in Transwell plates and incubated for 7, 14, or 21 days. Glycerol alone or a combination of glycerol and HgCl₂ was added to the apical side of the Transwell plate. The concentration of glycerol on the basal side was measured, and the P_app was then calculated. The data are presented as the mean ± SD for six experiments. Dunnett’s test: * p < 0.05 and ** p < 0.01 vs. the day 7 value.