Table 2.
Effect of inhibition of AQP3 function on the Papp of warfarin.
| Condition | Warfarin (10−6 cm/s) | Warfarin + HgCl2 (10−6 cm/s) |
|---|---|---|
| Normal condition | 25.2 ± 4.7 | 24.2 ± 1.9 |
| Osmotic pressure difference condition | 24.1 ± 2.2 | 24.2 ± 4.4 |
| Stirring condition | 54.6 ± 1.8 | 53.3 ± 5.4 |
Caco-2 cells were plated in Transwell plates and incubated for 21 days. (1) Normal condition: Warfarin alone or a combination of warfarin and HgCl2 was added to the apical side of the Transwell plate. (2) Osmotic pressure difference condition: Warfarin alone or a combination of warfarin and HgCl2 was added to the apical side of the Transwell plate, and mannitol was added to the basal side. (3) Stirring condition: Warfarin alone or a combination of warfarin and HgCl2 was added to the apical side, and the Transwell plate was shaken on a plate mixer. The concentration of warfarin on the basal side was measured, and the Papp was then calculated. The data are presented as the mean ± SD for six experiments.