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. 2019 Mar 28;20(7):1559. doi: 10.3390/ijms20071559

Table 3.

HPLC conditions.

Drug Mobile Phase Wavelength (nm) Flow Rate (mL/min) Retention Time (min)
Warfarin 20 mM phosphate buffer:methanol = 55:45 308 1.0 10.0
Antipyrine 20 mM phosphate buffer:methanol = 70:30 254 1.0 12.9
Atenolol 50 mM phosphate buffer:acetonitrile = 90:10 226 1.0 5.2
Furosemide 50 mM phosphate buffer:methanol = 60:40 280 1.2 6.7