Table 2.
Selected primers designed for molecular analysis of Verticillium spp.
| Targeted Organism (Number of Strains Analyzed) | Assay | Marker | Primers Sequences 5′-3′ | Primers Authors | Primers Used in |
|---|---|---|---|---|---|
|
V. albo-atrum (5) V. tricorpus (7) |
multi-plex PCR | ITS 1 | CCGGTCCATCAGTCTCTCTG CTGTTGCCGCTTCACTCG |
[79] | [79] |
| V. longisporum (42) | EF 2 | AAGTGGAGCCCCGTATCTTGAAT CAACTGGCAACAGGGCTTGAAT |
|||
| V. isaacii (14) | CGATGTCGCGATGACCTCG CGGCAGCCTCCTAAACATGG |
||||
| V. klebahnii (7) | ACATCCTGAGGCTGCTTGAGA CGGCAGCCTCCTAAACATGG |
||||
| V. zaregam- sianum (10) | GPD 3 | GGTTTCCTCCCCTCACACG CCACCCTTGATGTGGGCGGA |
|||
| V. longisporum (42) | CCCCGGCCTTGGTCTGAT TGCCGGCATCGACCTTGG |
||||
| V. alfalfae (7) | TCATGCCCCCTTTGTTCATCGAT TGCCGGCATCGACCTTGG |
||||
| V. albo-atrum (5) | ACT | GGCCTCGATAGCATCGCC CTGGATGGAGACGTAGAAGGC |
|||
| V. tricorpus (5) | CGTGCTGTCTTCCGTAAGTTTG CTGGATGGAGACGTAGAAGGC |
||||
| V. nonalfalfae (9) | TS 5 | CCTCGAAAAATCCACCAGCTCTA GTGGTTGAGATCCTCACGCTTC |
|||
| V. nubilum (4) | GGTCCCCCTCGTTCATGCAATC GTGGTTGAGATCCTCACGCTTC |
||||
| V. dahliae (10) V. longisporum (10) | PCR | ITS 1 | GGA AGTAAAAGTCGTAACAAGG TCCTCCGCTTATTGATATGC |
[91] | [19] |
| COX3 6 | TGATTTAGAGATST AATATCAGAAG CCGTGGAAACCTGTSCCAAAATA |
[19] | |||
| NAD1 7 | ATGGCSAGTATGCAAAGAAGA GCATGTTC TGTCATAAASCCACTAAC |
[92] | |||
| Verticillium spp. (7) | qPCR | ITS 1 | CTTGGTCATTTAGAGGAAGTAA AAAGTTTTAATGGTTCGCTAAGA |
[80] | [80] |
| V. tricorpus (4) | qPCR | ITS 1 | CCGGTGTTGGGGATCTACT GTAGGGGGTTTAGAGGCTG |
[91] | [83] |
| V. dahliae (9) + V. longisporum | β-tubulin | GGCCAGTGCGTAAGTTATTCT ATCTGGTTACCCTGTTCATCC |
[84] | ||
| V. longisporum (11) | β-tubulin | GCAAAACCCTACCGGGTTATG AGATATCCATCGGACTGTTCGTA |
|||
| V. dahliae (1) + V. longisporum (1) | qPCR | ITS 1 | CAGCGAAACGCGATATGTAG GGCTTGTAGGGGGTTTAGA |
[93] | [93] |
| V. dahliae (44) | qPCR | IGS 8 | CGTTTCCCGTTACTCTTCT GGATTTCGGCCCAGAAACT |
[87] | [87] |
| V. tricorpus (13) | endochitinase | TAGTAGAATACTAGATARCTAG AGCCTAGGTCTTTATAGCTAG |
|||
| V. dahliae | CTCGGAGGTGCCATGTACTG ACTGCCTGGCCCAGGTTC |
||||
| V. dahliae | β-tubulin | GCGACCTTAACCACCTCGTT CGCGGCTGGTCAGAGGA |
|||
| G3PD 9 | CACGGCGTCTTCAAGGGT CAGTGGACTCGACGACGTAC |
||||
| VTP1 10 | GCGGTGGCTGGTTCCTATCAAC CAACGACTTCGCCATCTGGAAG |
||||
| V. albo-atrum group 1 (isolation from soil) | qPCR | actin | GCCCTCTTCCAGCCCTCCGTTCTC TCGGCGTGGTTTTGTGGTGAG |
[87] | [94] |
| V. albo-atrum group 2 (isolation from soil) | qPCR | IGS 8 | CGTGTTTAGTGTATTTCACCCTTG TCGCAGAGTAGTACGATTTCTC |
[94] | |
| V. longisporum (isolation from soil) | CGAGGAGTGAAAAGAAAACGGTTA CGCGCCGAGGCTAGTCAC |
||||
| V. dahliae (5) | qPCR | not explained in the study | TCCTAGGCAGGCGAGCAG TAGGGCTGTCTGTCGGTGA |
[90] | [90] |
| V. albo-atrum (4) | TTTCACGACCGATGAAAGCG CACATCGGCGAGGATCTGTC |
||||
| V. tricopus (4) | CACCCTCGGGCACACCAATA TCCGTGGAGGTTGAGCGCTAT |
||||
| V. longisporum (4) | CGAGGAGTGAAAAGAAAACGGTTA CGCGCCGAGGCTAGTCAC |
1 internal transcribed spacer, 2 elongation factor 1-α, 3 glyceraldehyde-3-phosphate dehydrogenase, 4 actine, 5 tryptophan synthase, 6 cytochrome oxidase subunit III, 7 NADH dehydrogenase subunit 1, 8 intergenic spacer of genomic DNA, 9 glyceraldehyde-3-phosphate dehydrogenase, 10 extracellular trypsin protease.