Table 3.
Selected primers designed for molecular analysis of Phytophthora spp.
| Targeted Organism (No. of Strains Analyzed) | Assay | Marker | Primers Sequences 5′-3′ | Primer Authors | Primers Used in |
|---|---|---|---|---|---|
| Phytophthora spp. (15 in [101]; 14 in [102]) | PCR | ITS 1 | GGAAGTAAAAGTCGTAACAAGG TCCTCCGCTTATTGATATGC |
[91] | [101,102] |
| Phytophthora spp. (51) | PCR | COX1 2 | GCGTGGACCTGGAATGACTA AGGTTGTATTAAAGTTTCGATCG |
[114] | [114] |
| COX2 3 | AAAAGAGAAGGTGTTTTTTATGGA GCAAAAGCACTAAAAATTAAATATAA |
||||
| P. ramorum | nested PCR | Spacer sequences between the COX 2 and COX1 gene | GTATTTAAAATCATAGGTGTAATTTG TGGTTTTTTTAATTTATATTATCAATG |
||
| P. nemorosa | AATAAAATTAATTTTAATATATAATTAG TATGTTTAATATCTGTAAATAATAG |
||||
| P. pseudo- syringae | CAGTTTCATTAGAAGATTATTTAC AAAATTGTTTGATTTTATTAAGTATC |
||||
| Phytophthora spp. (82) | PCR | 60S ribosomal protein L10 | GCTAAGTGTTACCGTTTCCAG ACTTCTTGGAGCCCAGCAC |
[106] | [106] |
| β-tubulin | GCCAAGTTCTGGGARGTSAT GCCAAGTTCTGGGARGTSAT |
||||
| Enolase | CTTTGACTCGCGTGGCAAC CCTCCTCAATACGMAGAAGC |
||||
| Heat shock protein 90 | GCTGGACACGGACAAGAACC CGTGTCGTACAGCAGCCAGA |
||||
| tigA gene fusion | TTCGTGGGCGGYAACTGG TCGTGGGCGGYAAYTGGAA GCCTACATCACGGAGCARA TCGCYATCAACGGMTTCGG CCGAAKCCGTTGATRGCGA GCCCCACTCRTTGTCRTACCAC |
||||
| EF 4 | GGTCACCTGATCTACAAGTGC CCTTCTTGTTCACCGACTTG |
||||
| P. infestans (1) | qPCR | GC-rich nuclear satellite DNA with unknown function | GCCAT CAAGACGTGCGAGA GCAGGGATTCGGGCATA |
[108] | [108] |
| P. citricola (1) | ITS 1 | TCAACCCTTTTAGTTGGGGGTC TTTAAAACAAAAAGCTACTAGCCCAGAC |
|||
| Phytophthora spp. (71) | qPCR | ITS 1 | TGCGGAAAGGATCATTACCACACC GCGAGCCTAGACATCCACTG |
[109] | [109] |
| P. colocasiae (49) | qPCR | YPT1 5 | GGTGTGGACTTTGTGAGTTTCAG AAGGGAGTTGGCACAACCATT |
[111] | [111] |
| TRP1 6 | AGCGCCTTAACGCTCCCT GAGCCCTTGAACCACTTGGG |
||||
| GPA1 7 | TTGGTGGCGTGTAGTCTGTG AGCTTCCGGTTGATGGTAGC |
||||
| Phytophthora spp. (15) | qPCR | YPT1 4 | ATGAACCCCGAATAGTRCGTGC TGTTSACGTTCTCRCAGGCG |
[115] | [115] |
| TRP1 6 | GAGGAGATCGCGGCGCAGCG GCGCACATRCCGAGVTTGTG |
||||
| GPA1 7 | GGACTCTGTGCGTCCCAGATG ATAATTGGTGTGCAGTGCCGC |
||||
| P. nicotianae (7) | qPCR | ITS 1 | CCTATCAAAAAAAAGGCGAACG TACACGGAAGGAAGAAAGTCAAG |
[112] | [112] |
| P. cactorum (7) | YPT1 5 | CATGGCATTATCGTGGTGTA GCTCTTTTCCGTCGGC |
1 internal transcribed spacer, 2 cytochrome oxidase subunit I, 3 cytochrome oxidase subunit II, 4 Elongation factor 1-α, 5 RAS-related protein, 6 phosphoribosylanthranilate isomerase, 7 G protein α-subunit.