Figure 4.

Pharmacological activation of TRPM8 with icilin induces energy expenditure in wild-type and Pirt^−/− mice. Energy expenditure of female chow-fed wild-type (WT) (A) and Pirt^−/− (KO) mice (B) after subcutaneous injections of icilin (2 μmol/kg) (grey) or saline control (black) (n = 8 per genotype) with respective area under the curve (AUC) after icilin and saline injections (0–90 min) (C) and AUC of locomotor activity in the same period (D). Data represent mean ± s.e.m. *P < 0.05, **P < 0.01 determined by an unpaired two-tailed Student's t-test comparing saline and icilin injections within genotype.