Figure 4.

Quantification of PD-L1⁺ cell subsets in different compartments of immune cell types in peripheral blood and correlation with clinical responses. (a) Dot plot graph representing the percentage of PD-L1⁺ cells within systemic CD11b^negative subsets quantified from fresh peripheral blood samples before the start of immunotherapies, in objective responders (OR, N = 9), non-responders (NOR, N = 24), and healthy donors (N = 7). (b) Within CD11b⁺ cell subsets. (c) Within CD11b⁺ CD14^negative subsets. (d) Within CD11b+ CD14+ subsets. Relevant statistical comparisons are indicated within each graph, by the Fisher’s exact test, considering as cut-off values the indicated with horizontal red dotted lines. Means ± standard deviations are shown within the dot plots. Green, patients with >40% of systemic memory CD4 T cells; Black, patients with <40% of systemic memory CD4 T cells; Violet, patients with stable disease.